Flavocytochromeb2-Based Enzymatic Method of L-Lactate Assay in Food Products
نویسندگان
چکیده
منابع مشابه
Flavocytochrome b2-Based Enzymatic Method of L-Lactate Assay in Food Products
L-lactate, a key metabolite of the anaerobic glycolytic pathway, plays an important role as a biomarker in medicine, in the nutritional sector and food quality control. For these reasons, there is a need for very specific, sensitive, and simple analytical methods for the accurate L-lactate measuring. A new highly selective enzymatic method for L-lactate determination based on the use of flavocy...
متن کاملIs lactate an undervalued functional component of fermented food products?
Although it has been traditionally regarded as an intermediate of carbon metabolism and major component of fermented dairy products contributing to organoleptic and antimicrobial properties of food, there is evidence gathered in recent years that lactate has bioactive properties that may be responsible of broader properties of functional foods. Lactate can regulate critical functions of several...
متن کاملEvaluation of an Enzymatic Method for Determining Serum Creatinine Assay
The enzymatic method for the determination of serum creatinine is accepted as one of the standard method in a clinical laboratory. The enzymatic method for the determination of serum creatinine was optimized for use with Merilyzer AutoQuant-400 auto analyzer and its performance characteristics were practically compared with Jaffe’s Kinetic. Effects of some interfering substances like Serum bili...
متن کاملAutomated enzymatic determination of L-lactate in serum, with use of a miniature centrifugal analyzer.
We describe an automated enzymatic reaction-rate method for spectrophotometric determination of lactate in serum with a miniature centrifugal analyzer. The L(+) -lactate is selectively oxidized in the presence of lactate dehydrogenase (EC 1.1.1.27) and NAD+ to form NADH, which is measured from its absorption. Reaction rates are determined automatically, and unknown concentrations are calculated...
متن کاملEffect of lactate dehydrogenase isoenzymes on the coupled enzymatic assay for alanine aminotransferase activity.
We show an example of the importance of specifying the form of isoenzyme and source of indicator enzymes to be used in coupled enzymatic assays. When we compared H-4 (pig heart) and M-4 (rabbit muscle) isoenzymes of lactate dehydrogenase for their suitability as indicator enzymes in the assay for alanine aminotransferase activity, we found that about fourfold as much M-4 as H-4 was required in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Scientific World Journal
سال: 2013
ISSN: 1537-744X
DOI: 10.1155/2013/461284